Mature Hairy Tube New !link! May 2026

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• Lag 0–3 days; exponential 4–18 days; stationary 19–28 days.
• Final FW: 4.2 ± 0.3 g per 50 mL culture; DW: 0.48 ± 0.04 g.

1. Confirm identity of target metabolites by LC-MS/MS and comparison to standards.
2. Test elicitors (methyl jasmonate, salicylic acid) and precursor feeding to boost yields.
3. Scale-up in temporary immersion or airlift bioreactors for higher biomass.
4. Maintain antibiotic selection to prevent Agrobacterium persistence; verify absence of bacteria by plating.

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