Clsi Document — M45 Pdf

CLSI M45

The document, titled Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria , is a specialized guideline essential for clinical microbiology laboratories handling organisms that are not covered by the standard CLSI M100 . Helpful Review & Key Highlights

Q: Does M45 cover mycobacteria or fungi?

A: No. See CLSI M24 (mycobacteria) and M61 (yeasts/molds). clsi document m45 pdf

• Acceptable for select organisms with established zone diameter breakpoints.
• Requires carefully prepared inoculum and specified agar (e.g., Mueller-Hinton with 5% sheep blood for Pasteurella).
• Not recommended for very slow growers (e.g., H. pylori).

• Campylobacter jejuni ATCC 33560
• Haemophilus influenzae ATCC 49247
• Neisseria gonorrhoeae ATCC 49226